Molecular Biology?

Question 1


An investigator would be able to distinguish a solution containing RNA from one containing DNA by:


a. Monitoring the change in absorption of light at 260 nm while elevating the temperature.


b. Heating the solutions to 82.5°C and measuring the absorption of light at 260 nm.


c. Comparing the Tm of each solution.


d. Measuring the absorption of light at 260 nm.





Question 2


What happens to a supercoiled, circular DNA molecule if it becomes nicked?


a. A relaxed circle forms.


b. A linear molecule forms.


c. It is degraded to nucleotide subunits.


d. Torsional stress increases.





Question 3


DNA replication:


a. Requires a DNA template, deoxynucleotides, primers, and DNA polymerase.


b. Can be initiated de novo.


c. Is conservative.


d. Proceeds directionally from 3’ to 5’.

Molecular Biology?
1. a


2. a (The nick allows the DNA to un-supercoil)


3. c (DNA goes 5' to 3')
Reply:1. a.


2. a.


3. c.