General Biology Question on DNA, mRNA and tRNA?

The following is a DNA strand formed by nucleotides.


3'-TAC CCA CTT CGA ACT- 5'





where


A - Adenine


T - Thymine


C - Cytosine


G - Guanine





1) A modelling kit, while useful in demonstrating the main steps of DNA replication, transcription of genetic information from DNA to mRTA, and translation of genetic information from mRNA to polypeptides, cannot completely mimic the same processes in a living cell. Describe three differences between what actually happens in a living cell and what had been done using the kit.





2) Would a base substitution nearer to the 3' end of the template DNA have a more harmful effect than a base substitution nearer to the 5' end of the template DNA? Explain your answer.

General Biology Question on DNA, mRNA and tRNA?
a sub at the 3 end would be worse because it could throw off the rest of the chain but that is just a guess.