Thursday, July 30, 2009

Genetics Multiple Choice Question: DNA polymerase I is thought to add nucleotides?

DNA polymerase I is thought to add nucleotides?


A. to the 5' end of the primer


B. to the 3' end of the primer


C. in the place of the primer RNA after it is removed


D. on single-stranded templates without need for an RNA primer


E. in a 5' to 5' direction

Genetics Multiple Choice Question: DNA polymerase I is thought to add nucleotides?
B. because as DNA pol I removes primer from the 5' end, it is replacing the primer with DNA at the 3" end (on the adjacent strand)





5'______________3'


3'______________5'
Reply:B
Reply:C.





Nucleotides are added from 3' of old strand towards 5' by DNA polymerase III.





DNA polymerase ! also adds nucleotides at Okazaki fragments.
Reply:B

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