Tuesday, July 28, 2009

The necessary ingredients for DNA synthesis can be mixed together in a test tube.?

The necessary ingredients for DNA synthesis can be mixed together in a test tube. The DNA polymerase is from Thermus aquaticus and the template is from a human cell. The DNA synthesized would be most similar to :


A.


Human DNA.


B.


T. aquaticus DNA.


C.


A mixture of human and T. aquaticus DNA.


D.


Human RNA.


E.


T. aquaticus RNA.

The necessary ingredients for DNA synthesis can be mixed together in a test tube.?
the answer is A, because all the DNA polymerase from the Thermus aquaticus does is copy the human DNA template. The reason why the thermus aquaticus is used is because this process, PCR, utilizes high temperatures which denatures normal proteins. Thermus aquaticus is a bacteria found in hot springs at Yellowstone National Park, and thus can withstand those temperatures.
Reply:A the sythesis reproduces the template DNA regardless of the enzymes used T. aquaticus is used as an enzyme source because it is easy to use in the lab. We just cycle temperatures to run the different phases of the reaction.


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