Thursday, July 30, 2009

Ok...two more bio Q's I need help on.?

I can't figure out these two:





First off:





In order to preserve cellular homeostasis, an important exchange pump within the cell membrane ejects __________ ions from the cell and imports ___________ ions.


a.2 potassium; 3 calcium


b.3 sodium; 2 calcium


c. 2 potassium; 3 sodium


d. 3 sodium; 2potassium


e. 2 calcium; 3 sodium





Secondly:((I NEED HELP FINDING THE AMINO ACID--I DONT KNOW HOW DO FIND THAT))


The following nucleotide sequence is the coding strand of DNA.


ATG ACT TGT GTC CTA TAG





Please provide the sequence of the template strand








Please transcribe the mRNA from this DNA








Please translate the amino acid sequence from this message





THANKS!!!!!

Ok...two more bio Q's I need help on.?
d)ejects 3 sodium ions and imports 2 potassium ions





Nucleic acid sequences are always written in the 5'-%26gt;3' direction





so you have the coding sequence:





5' -ATG ACT TGT GTC CTA TAG -3'





The template sequence you can find easily by pairing A with T, T with A, G with C and C with G





5' -ATG ACT TGT GTC CTA TAG -3'


3' -TAC TGA ACA CAG GAT ATC -5'





Now, for a not very advanced class the professor would be content with the answer TAC TGA ACA CAG GAT ATC as the template sequence. However that would be wrong, since nucleic acid sequences have to be written in the 5'-%26gt;3' direction so the truly correct answer is:


5'-CTA TAG GAC ACA AGT CAT-3'





mRNA has the same sequence as the coding DNA with only difference that you have U instead of T:


5' -AUG ACU UGU GUC CUA UAG -3'





You need a table with the code. You can also use on-line tools like: http://au.expasy.org/tools/dna.html


M T C V L Stop or if you prefer 3-letter code





Met Thr Cys Val Leu stop (well stop is not part of the protein but its just to show that the last codon is a stop codon)


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