Thursday, July 30, 2009

Molecular/Cell Biology...need help please!?

One strand of a section of DNA isolated from the bacterium E. coli reads:


5'-gtagcctacccatagg-3'


a)suppose that an mrna is transcribed from this dna using the complementary strand as a template. What will be the sequence of the mrna in this region


b)how many different peptides could potentiallybe made from this sequence of rna, assuming translation initiates upstream of this sequence?Why?


c)what are these peptides?(answer using one letter amino acid codes)

Molecular/Cell Biology...need help please!?
a) GUAGCCUACCCAUAGG, because the sequence would be the same because you would be transcribing the complementary strand





b) 3, because there are three possible reading frames





c) Frame 1: V-A-Y-P-Stop (codons: GUA-GCC-UAC-CCA-UAG)


Frame 2: the first amino acid would depend on the immediately preceding two letters-STOP (codons:XXG-UAG)


Frame 3: the first amino acid would depend on the immediately preceding letter-S-L-P-I-G (codons: XGU-AGC-CUA-CCC-AUA-GGX)


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